As a study director, Sandra works routinely on pre-clinical models such as PAH and COPD. Over the past three years she and her team have developed the implantation method of pressure telemetry probes in rats.
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CCI-4-induced Liver fibrosis
Purpose
The CCl4-induced model of liver fibrosis is a widely used and studied, reliable animal model of hepatic fibrosis. Repeated doses of CCl4 lead to repeated rounds of wound-healing, causing hepatic stellate cells activation, imbalances between extracellular matrix production and degradation, and development of progressive hepatic fibrosis.
Method
Liver fibrosis will be induced by CCl4 injection; CCl4 will be mixed with olive oil (10% solution) and intraperitonealy administration will be given twice weekly for 6 weeks.
Study outcome
- Plasmatic liver inflammation biomarkers (ALP, ALT, ASP, bilirubin)
- Liver oxidative stress marker (MDA, lipid peroxidation)
- Histopathology of formalin-fixed and paraffin-embedded liver section
- H&E staining for morphology and steatosis
- Masson’s trichrome for fibrosis
- Sirius red for collagen content
Species
Mice and rats
Compliance
Non-GLP compliant. The study is best suited to demonstrate efficacy in discovery-phase preclinical development strategies.
Sandra Gagnon
Study Director
