CCI-4-induced Liver fibrosis


The CCl4-induced model of liver fibrosis is a widely used and studied, reliable animal model of hepatic fibrosis. Repeated doses of CCl4 lead to repeated rounds of wound-healing, causing hepatic stellate cells activation, imbalances between extracellular matrix production and degradation, and development of progressive hepatic fibrosis.



Liver fibrosis will be induced by CCl4 injection; CCl4 will be mixed with olive oil (10% solution) and intraperitonealy administration will be given twice weekly for 6 weeks.

Study outcome

  • Plasmatic liver inflammation biomarkers (ALP, ALT, ASP, bilirubin)
  • Liver oxidative stress marker (MDA, lipid peroxidation)
  • Histopathology of formalin-fixed and paraffin-embedded liver section
  • H&E staining for morphology and steatosis
  • Masson’s trichrome for fibrosis
  • Sirius red for collagen content


Mice and rats



Non-GLP compliant. The study is best suited to demonstrate efficacy in discovery-phase preclinical development strategies.

Sandra Gagnon

Study Director

As a study director, Sandra works routinely on pre-clinical models such as PAH and COPD. Over the past three years she and her team have developed the implantation method of pressure telemetry probes in rats.