FeCl3-induced Arterial Thrombosis

Introduction
Ferric chloride (FeCl3)-induced thrombus formation is a widely used experimental model for arterial thrombosis. Tissue damage initiated by iron-mediated chemical oxidation predisposes the injured area to platelet adherence and aggregation followed by coagulation activation and fibrin deposition.

Methodology
A strip of filter paper soaked with FeCl3 is placed around the vessel for a short period of time to induce arterial injury and thrombosis. Arterial blood
flow is monitored and the time to occlusion (TTO) is measured.

Readouts

• TTO
• Histology
• Bleeding time
• Platelet aggregation, thromboelastogram

Application
This model is perfectly suited to assess the anti-coagulant/ anti-platelet efficacy of a new drug entity.